HPLC Column, Bidentate C8 2.o, 2.2um, 2.1mm ID x 50mm Length, 120A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40208‑05P‑2 Cogent Bidentate C8™ 2.o HPLC Column is a short, narrow‑bore, high‑efficiency reversed‑phase column designed for applications where C18 chemistry is overly retentive and alternative selectivity is required. Built on Cogent TYPE‑C™ silica hydride technology, this column combines smaller 2.2 µm particles with bidentate C8 bonding to deliver fast separations, excellent efficiency, and distinctive selectivity—even on standard HPLC systems.
The Bidentate C8™ 2.o stationary phase is particularly effective for hydrophobic and non‑polar compounds that are difficult to retain in aqueous mobile phases, but that may be excessively retained on C18 columns. The shorter alkyl chain reduces hydrophobic interaction strength while maintaining the chemical robustness and low silanol activity characteristic of TYPE‑C™ silica hydride phases.
The 2.1 mm internal diameter and 50 mm length enable rapid run times, reduced solvent consumption, and enhanced mass‑spectrometric sensitivity. As with all narrow‑bore columns, optimal performance requires precise low‑flow pumping and detector flow cells designed for reduced internal volumes, making this column a favorite choice for LC‑MS workflows.
Why Choose Bidentate C8™ 2.o Instead of C18?
The Bidentate C8™ 2.o is selected when:
C18 columns provide excessive retention or long run times
Non‑polar compounds require controlled retention under aqueous conditions
Faster equilibration and shorter gradients are desired
Alternative reversed‑phase selectivity improves separation outcomes
Coupled with TYPE‑C™ silica hydride chemistry, the C8 ligand provides a broader method window than conventional C8 phases.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Reduced silanol activity and secondary interactions
Faster equilibration than traditional silica‑based C8 columns
Excellent chemical and pH stability
Bidentate C8™ 2.o Bonding
Two‑point attachment via direct Si–C bonds
Alternative selectivity when C18 is overly retentive
Maintains retention of hydrophobic analytes with better control
Near‑UHPLC Efficiency (2.2 µm)
High efficiency on standard HPLC instruments
Improved resolution and peak capacity
Suitable for demanding analytical and screening workflows
LC‑MS‑Optimized Geometry
2.1 mm ID enhances sensitivity and reduces solvent use
50 mm length supports fast analysis
Ideal for ESI‑MS and LC‑MS/MS
System Requirements
Because of the narrow‑bore, small‑particle format, optimal performance requires:
A pump capable of precise, stable low‑flow delivery
A detector flow cell designed for reduced internal volumes
Systems not designed for low‑flow operation may not achieve full efficiency.
Typical Applications
LC‑MS and LC‑MS/MS
Hydrophobic and non‑polar compounds
Situations where C18 retention is excessive
Rapid method development and screening
High‑efficiency reversed‑phase separations